Sargassum fusiforme and its fucoidan alleviates high-fat diet induced obesity associated with the improvement of the gut microbiota profile by Xiaoyu Ling, Zhengyang Li, Ning Su, Lingqing Kong, Shiwei Han, Dafeng Song

“…This study investigates the mechanisms by exploring changes in gut microbiota composition, gene expression, and metabolites in mice fed a high-fat diet after intervention with Sargassum fusiforme (SF) and Sargassum fusiforme fucoidan (SFF) through integration of 16S rRNA sequencing, transcriptomics, and non-targeted metabolomics. …”
Get full text

Integrated multi-omics analysis reveals the underlying molecular mechanism for the neurotoxicity of triclosan in zebrafish by Shasha Zhao, Yuhang Ling, Baohua Zhang, Danting Wang, Limei Sun

“…Transcriptomics indicated that immune- and metabolism-related gene expression patterns were severely disturbed by TCS. …”
Get full text

Impact of Paracoccus sp. EGY7 carotenoids on triple-negative breast cancer cells: invitro study by Karim Abdelazim, Ahmed Hussein, Sherine N. Khattab, Shaymaa Essam El Feky, Nehad Noby

“…The extract induced apoptosis, as evidenced by significantly increased BAX/BCL-2 gene expression ratios at 600 and 1200 µg (p < 0.05). …”
Get full text

Comprehensive landscape and oncogenic role of extrachromosomal circular DNA in malignant biliary strictures by Zhuo Cheng, Xuanmei Luo, Wenzheng Liu, Xiaofang Lu, Hong Chang, Yingchun Wang, Wei Zheng, Xiue Yan, Yonghui Huang

“…EccDNA carrying miR-106a/363 cluster or miR-374b/421 cluster were proven to regulate cancer gene expression, accelerate tumor proliferation, and inhibit tumor apoptosis. …”
Get full text

Lower urinary dysfunction as a long-term effect of childhood vincristine treatment, with potential influences by sex and dose by Nao Iguchi, Ali Teimouri, Duncan T. Wilcox, Anna P. Malykhina, Nicholas G. Cost

“…Significant changes in gene expression levels associated with neuroinflammation and nociception were observed in both the bladder and lumbosacral dorsal root ganglia (Ls-DRG) of the VCR group. …”
Get full text

Multi-locus CRISPRi targeting with a single truncated guide RNA by Molly M. Moore, Siddarth Wekhande, Robbyn Issner, Alejandro Collins, Anna J. Cruz, Yanjing V. Liu, Nauman Javed, Salvador Casaní-Galdón, Jason D. Buenrostro, Charles B. Epstein, Eugenio Mattei, John G. Doench, Bradley E. Bernstein, Noam Shoresh, Fadi J. Najm

“…Abstract A critical goal in functional genomics is evaluating which non-coding elements contribute to gene expression, cellular function, and disease. Functional characterization remains a challenge due to the abundance and complexity of candidate elements. …”
Get full text

PPIH is a novel diagnostic biomarker associated with immune infiltration in cholangiocarcinoma by Jun Ye, Zhitao Chen, Chuan Zhang, Rui Xie, Haini Chen, Peng Ren

“…Methods We analyzed the expression levels, prognostic significance, and diagnostic efficiency of PPIH in CHOL using data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets, coupled with gene enrichment analyses. …”
Get full text

Single-cell landscape of dynamic changes in CD8+ T cells, CD4+ T cells and exhausted T cells in hepatocellular carcinoma by Rongqiang Liu, Jing Ye, Jianguo Wang, Wangbin Ma, Zhendong Qiu, Jia Yu, Weixing Wang

“…Finally, immunohistochemistry and real-time PCR were used to verify gene expression. The hdWGCNA algorithm identified 40 genes strongly associated with CD4+ T cells, CD8+ T cells and exhausted T cells. …”
Get full text

Comprehensive genome-wide analysis of ARF transcription factors in orchardgrass (Dactylis glomerata): the positive regulatory role of DgARF7 in drought resistance by Miaoli Wang, Wen Liu, Guangyan Feng, Gang Nie, Zhongfu Yang, Feixiang Hao, Linkai Huang, Xinquan Zhang

“…DgARF7 also accelerated flowering time by inducing the flowering-related gene expression levels in Arabidopsis. This research provides important insights into the role of DgARF7 in orchardgrass and provides further understanding in molecular breeding.…”
Get full text

Role of exercise on ncRNAs and exosomal ncRNAs in preventing neurodegenerative diseases: a narrative review by Shangwu Liu, Runhong Zhang, Jamal Hallajzadeh

“…Non-coding RNAs (ncRNAs) and exosomal ncRNAs associated with vesicles have been recognized as influencers of gene expression and cell signaling, potentially contributing to the positive impact of physical activity on neurodegenerative conditions. …”
Get full text

Tire rubber-derived contaminants 6PPD and 6PPD-quinone reduce attachment and outgrowth of trophoblast spheroids onto endometrial epithelial cells by Jong Geol Lee, Seon Min Lee, Moonjung Hyun, Jeong Doo Heo

“…A similar result was observed in 1 μM 6PPDQ-exposed groups. Gene expression profiling of 6PPD- and 6PPDQ-exposed endometrial epithelial cells revealed that both 6PPD and 6PPDQ differentially regulated a panel of transcript markers toward overall downregulation of receptivity and invasion. …”
Get full text

Machine learning-random forest model was used to construct gene signature associated with cuproptosis to predict the prognosis of gastric cancer by Xiaolong Liu, Pengxian Tao, He Su, Yulan Li

“…Transcriptome and clinical data of patients with GC were collected from The Cancer Genome Atlas and Gene Expression Omnibus datasets. Single sample gene set enrichment analysis (GSEA) and the randomized forest method were used to establish the prognostic model. …”
Get full text

Acidovorax temperans skews neutrophil maturation and polarizes Th17 cells to promote lung adenocarcinoma development by Joshua K. Stone, Natalia von Muhlinen, Chenran Zhang, Ana I. Robles, Amy L. Flis, Eleazar Vega-Valle, Akihiko Miyanaga, Masaru Matsumoto, K. Leigh Greathouse, Tomer Cooks, Giorgio Trinchieri, Curtis C. Harris

“…These T17 cells shared a common gene expression program predictive of poor survival in human LUAD. …”
Get full text

Structural basis of SIRT7 nucleosome engagement and substrate specificity by Carlos Moreno-Yruela, Babatunde E. Ekundayo, Polina N. Foteva, Dongchun Ni, Esther Calvino-Sanles, Henning Stahlberg, Beat Fierz

“…Abstract Chromatin-modifying enzymes target distinct residues within histones to finetune gene expression profiles. SIRT7 is an NAD+-dependent deacylase often deregulated in cancer, which deacetylates either H3 lysine 36 (H3K36) or H3K18 with high specificity within nucleosomes. …”
Get full text

The effect of drought stress on Pinellia ternata in terms of physiology and aquaporin genes after Arbuscular mycorrhizal fungal inoculation by Shuang Li, Yajun Liu, Han He, Yike Xu, Xiaochun Sun, Wenjing Huang

“…AMF can alleviate drought stress-induced damage by regulating photosynthetic capacity, osmosis, antioxidant activities, and aquaporin gene expression. Our study highlights the effect of AMF on P. ternata under drought stress by mediating physiological and biochemical activities. …”
Get full text

Emerging Anthelmintic Resistance in Poultry: Can Ethnopharmacological Approaches Offer a Solution? by Zirintunda, Gerald, Biryomumaisho, Savino, Keneth Iceland, Kasozi, Batiha, Gaber El-Saber, Kateregga, John, Vudriko, Patrick, Nalule, Sarah, Olila, Deogracious, Mariam Kajoba, Mariam, Matama, Kevin, Kwizera, Mercy Rukundo, Ghoneim, Mohammed M., Abdelhamid, Mahmoud, Alshehri, Sultan, Abdelgawad, Mohamed A., Acai-Okwee, James, Zaghlool, Sameh S.

“…Important phenolic acids have been found in Brassica rapa L. and Terminalia avicenniodes Guill. and Perri that affect the cell signaling pathways and gene expression. Benzo (c) phenanthridine and isoquinoline alkaloids are neurotoxic to helminths. …”
Get full text

Evaluation of Anti-Cancer, Pro-Apoptotic, and Anti-Metastatic Effects of Synthetic Analogue Compound 2-Imino-7-Methoxy-4-(4-fluorophenyl)-2H-1,3-Thiazino [3,2-a] Benzimidazole on P... by Mpho Ndou, Marthe C. D. Fotsing, Michael H. K. Kengne, Edwin M. Mmutlane, Derek T. Ndinteh, Mthokozisi B.C. Simelane, Lesetja Motadi, Mpho Choene

“…Caspase 3/7 activation was evaluated using caspase-Glo® 3/7 substrate reagent. Gene expression was analyzed using conventional polymerase chain reaction (PCR). …”
Get full text

Differentially expressed transcript isoforms associate with resistance to tuberculin skin test and interferon gamma release assay conversion. by Jason D Simmons, R Max Segnitz, Kimberly A Dill-McFarland, Catherine M Stein, Glenna J Peterson, Harriet Mayanja-Kizza, W Henry Boom, Thomas R Hawn

“…<h4>Materials and methods</h4>Monocytes from 49 RSTR versus 52 subjects with latent Mtb infection (LTBI) were infected with M. tuberculosis (H37Rv) or left unstimulated (media) prior to RNA isolation and sequencing. RSTR-associated gene expression was then identified using differential transcript isoform analysis.…”
Get full text

Vitrification affects the post-implantation development of mouse embryos by inducing DNA damage and epigenetic modifications by Yurong Chen, Haibo Zhu, Fucheng Guo, Luyao Wang, Wenli Zhang, Ruizhi Liu, Xiaoling Zhang, Xiangpeng Dai

“…Transmission electron microscopy (TEM) analysis was performed to exam the mitochondrial ultrastructure in blastocysts. The related gene expression and transcriptome profiles were investigated by RT-qPCR and RNA-seq, respectively. …”
Get full text