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    Protocol for predicting the single-cell network-based gene activity landscape during human B cell development by Xin Huang, Xuetong Hou, Yizhen Li, Jun J. Yang, Jiyang Yu

    Published 2025-03-01
    “…Summary: Owing to inconsistencies in human B cell classification and the difficulty in distinguishing heterogeneous subpopulations, we present a protocol to construct gene regulatory networks and gene activity landscapes for human B cell developmental stages. …”
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  3. 223

    Enhancing salinity tolerance in cultivated rice through introgression of African rice genes and application of moringa leaf extract by Heba A. Saleh, Shaimaa M. N. Tourky, Farag Ibraheem, Samy A. Abo-Hamed, Wafaa M. Shukry, Walid H. Elgamal, Eman M. Elghareeb

    Published 2025-02-01
    “…Utilizing wild rice-derived genes and biostimulants with high growth promoting- and stress-alleviating potential can significantly improve salinity tolerance in cultivated rice. …”
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    Integration of transcriptomics and metabolomics data revealed role of insulin resistant SNW1 gene in the pathophysiology of gestational diabetes by Ravi Bhushan, Rimjhim Trivedi, Ritu Raj, Anjali Rani, Sangeeta Rai, Anima Tripathi, Shiv Govind Rawat, Ajay Kumar, Dinesh Kumar, Pawan K. Dubey

    Published 2025-02-01
    “…This study identified SNW1 as a novel insulin-resistant gene that induces hyperglycemia by elevating gluconeogenesis and decreasing glucose uptake. …”
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    A cross-tissue transcriptome-wide association study identified susceptibility genes for age-related macular degeneration by Hongfan Yang, Haofei Huang, Kunlin Pu

    Published 2025-02-01
    “…A transcriptome-wide association study (TWAS) was conducted by integrating eQTL data from 49 tissues of the Genotype-Tissue Expression Project (GTEx) v8 and AMD data from FinnGen R10. The Unified Test for Molecular Signatures (UTMOST) and Functional Summary-based Imputation (FUSION) were used to evaluate gene associations with AMD across tissues and within individual tissues, respectively. …”
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  10. 230

    Machine Learning Diagnostic Model for Hepatocellular Carcinoma Based on Liquid–Liquid Phase Separation and Ferroptosis-Related Genes by Wenchao Chen, Ting Zhu, Xiaofan Pu, Linlin Zhao, Senhao Zhou, Xin Zhong, Suihan Wang, Tianyu Lin

    Published 2025-02-01
    “…Materials and Methods: The differentially expressed genes (DEGs) were identified based on 3 GEO datasets, followed by overlapping with LLPS-related and ferroptosis-related genes. …”
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  11. 231

    Genome-wide transcriptome analysis and drug target discovery reveal key genes and pathways in thyroid cancer metastasis by Minjing Zou, Amal Qattan, Monther Al-Alwan, Hazem Ghebeh, Naif Binjumah, Latifa Al-Haj, Khalid S. A. Khabar, Abdulmohsen Altaweel, Falah Almohanna, Abdullah M. Assiri, Abdelilah Aboussekhra, Ali S. Alzahrani, Ali S. Alzahrani, Yufei Shi

    Published 2025-02-01
    “…MethodsWe performed genome-wide transcriptome analysis comparing gene expression profile of metastatic thyroid cancer cells (Met) with primary tumor cells established from transgenic mouse models of papillary thyroid cancer (PTC), follicular thyroid cancer (FTC), poorly differentiated thyroid cancer (PDTC), and anaplastic thyroid cancer (ATC).ResultsGenes involved in tumor microenvironment (TME), inflammation, and immune escape were significantly overexpressed in Met cells. …”
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  12. 232

    Annotation-free deep learning for predicting gene mutations from whole slide images of acute myeloid leukemia by Bo-Han Wei, Xavier Cheng-Hong Tsai, Kuo-Jui Sun, Min-Yen Lo, Sheng-Yu Hung, Wen-Chien Chou, Hwei-Fang Tien, Hsin-An Hou, Chien-Yu Chen

    Published 2025-02-01
    “…Despite the demonstrated potential for characterizing gene mutations directly from WSIs in certain cancers, challenges remain due to image resolution and reliance on manual annotations for acute myeloid leukemia (AML). …”
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  13. 233

    A comprehensive transcriptional reference for severity and progression in spinal cord injury reveals novel translational biomarker genes by Rubén Grillo-Risco, Marta R. Hidalgo, Beatriz Martínez-Rojas, Victoria Moreno-Manzano, Francisco García-García

    Published 2025-02-01
    “…This study characterized distinct SCI phases and severities at the gene and functional levels, focusing on biomarker gene identification. …”
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    Integrating Bulk and Single-Cell Transcriptomic Data to Identify Ferroptosis-Associated Inflammatory Gene in Alzheimer’s Disease by Zhou H, Peng Y, Huo X, Li B, Liu H, Wang J, Zhang G

    Published 2025-02-01
    “…However, the cellular and molecular links between ferroptosis, immune inflammation, and the brain-peripheral blood axis in Alzheimer’s disease (AD) remain unclear.Methods: We integrated bulk RNA-seq data from AD brain tissue and peripheral blood and refined the screening of AD candidate genes through differential gene expression analysis, weighted gene co-expression network analysis (WGCNA), and other approaches. …”
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  17. 237

    Inhibition of complement system-related gene ITGB2 attenuates epithelial–mesenchymal transition and inflammation in diabetic nephropathy by Jun Peng, Wenqi Zhao, Lu Zhou, Kun Ding

    Published 2025-02-01
    “…Methods DN microarray datasets were downloaded from the GEO database, followed by differentially expressed genes (DEGs) screening. …”
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  18. 238

    Caveolin Gene, a Possible Risk Factor for Metabolic Syndrome in Humans: A Systematic Review and Meta-Analysis by Mohadeseh Arefian, Sadegh Mazaheri-Tehrani, Maryam Yazdi, Roya Kelishadi

    Published 2025-01-01
    “…Background: Studies show that caveolin genes are associated with metabolic disorders, so we aimed to systematically review the association between caveolin genes and metabolic syndrome in human studies. …”
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    Vegetation restoration effectiveness with main factors in the Beijing-Tianjin sandstorm source region during 2000-2020, China. by Xingshuo Zhang, Jingfang Yuan, Xiaoman Liu, Cheng Zong

    Published 2025-01-01
    “…The Beijing-Tianjin Sandstorm Source Region (BTSSR), a region with significant vegetation degradation in China, has been subjected to ecological engineering intended to curb vegetation browning. …”
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