A guide to selecting high-performing antibodies for Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (PPP2R5D) for use in Western Blot, immunoprecipi... by Carl Laflamme, Kathleen Southern, Riham Ayoubi, Maryam Fotouhi, Charles Alende

“…Pathogenic mutations in the PPP2R5D gene are linked to clinical symptoms characterized by neurodevelopmental delay, intellectual disability, and autism spectrum disorders. …”
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Induced hepatocyte-like cells derived from adipose-derived stem cells alleviates liver injury in mice infected with Echinococcus Multilocularis by Hui Liu, Xiaojuan Bi, Ning Yang, Xue Zhang, Bingbing Fang, Nuerbaiti Kusuman, Wenmei Ma, Jing Li, Jin Chu, Li Sun, Liang Li, Guodong Lü, Renyong Lin

“…The iHEPs exhibit morphological features and a glycogen storage function similar to those of mature hepatocytes and demonstrate an upregulation in hepatic gene programs with increasing induction time. Following transplantation, iHEPs were observed surrounding the metacestode lesions in the liver parenchyma of E. multilocularis-infected mice. iHEPs transplantation effectively restored liver function and improved liver injury in the infected mice. …”
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OsHMA3 overexpression works more efficiently in generating low-Cd rice grain than OsNramp5 knockout mutation by Yuejing Gui, Joanne Teo, Dongsheng Tian, Raji Mohan, Zhongchao Yin

“…Pyramiding the OsNramp5 mutant allele and the OsHMA3 transgene in a single line did not result in an additional reduction in grain Cd content. The OsNramp5 gene in T5105 has a haplotype II promoter, and its knockout mutation also partially reduced Mn content in rice grain. …”
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The role of candidate genetic polymorphisms in covid-19 susceptibility and outcomes by Anthony Yazbeck, Reem Akika, Zainab Awada, Nathalie K. Zgheib

“…Conclusion This study highlights the importance of genetic testing for key host genes involved in COVID-19 life cycle and eventually measuring the PRS which proves to be an important tool for prognosis assessment in vulnerable individuals, potentially enhancing patient care.…”
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Inhibition of STING-mediated type I IFN signaling by African swine fever virus DP71L by Lakmal Ranathunga, Sachini Abesinghe, Ji-Won Cha, Niranjan Dodantenna, Kiramage Chathuranga, Asela Weerawardhana, D. K. Haluwana, Nuwan Gamage, Jong-Soo Lee

“…Consistently, knockdown of DP71L enhanced antiviral gene expression in ASFV-infected cells. Taken together, these results highlight the important role of DP71L with respect to inhibition of interferon responses and provide guidance for a better understanding of ASFV pathogenesis and the development of live attenuated ASFV vaccines.…”
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Integrated multi-omics analysis reveals the underlying molecular mechanism for the neurotoxicity of triclosan in zebrafish by Shasha Zhao, Yuhang Ling, Baohua Zhang, Danting Wang, Limei Sun

“…Transcriptomics indicated that immune- and metabolism-related gene expression patterns were severely disturbed by TCS. …”
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Cannabidiol attenuates lipid metabolism and induces CB1 receptor-mediated ER stress associated apoptosis in ovarian cancer cells by Xuanhe Fu, Zhixiong Yu, Fang Fang, Weiping Zhou, Yuxin Bai, Zhongjia Jiang, Biao Yang, Ye Sun, Xing Tian, Guangyan Liu

“…Fatty acid metabolic profiling and flow cytometry analysis revealed that CBD has the ability to decrease fatty acid levels and significantly suppress the transcription of genes involved in fatty acid uptake and synthesis in ES-2 cells. …”
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BP-3 exposure at environmentally relevant concentrations induced male developmental reproductive toxicity via ER/CCL27/ROS pathway in mice by Hong-Mei Li, Yan-Rong Gao, Qing Chang, Xiu-Ying Pei, Jia-He Sun, Yu-Jia Lin, Ya-Nan Tian, Qiang-Wang, Bin Zhao, Heidi Qunhui Xie, Hui-Ming Ma, Hai-Ming Xu

“…In vitro, si-CCL27 and/or ROS scavenger treatment significantly antagonized BP-3-induced oxidative stress and the decrease of BTB and cytoskeleton related genes in TM4 cells. These findings demonstrate that prolonged exposure to low-doses of BP-3 resulted in detrimental effects on testicular development through activation of the ER/CCL27/ROS axis. …”
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Diagnostic accuracy of high-resolution melting curve analysis for discrimination of oncology-associated mutations: a systematic review and meta-analysis by Shu Yu, Yan Cheng, Chen-Cheng Tang, Yue-Ping Liu

“…Objective To investigate the diagnostic value of high-resolution melting (HRM) analysis for oncology-associated epidermal growth factor receptor ( EGFR) gene mutations. Methods We systematically searched Embase, PubMed, and Web of Science for HRM and EGFR mutation detection studies published through September 2024. …”
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Bacteriocin-like peptides encoded by a horizontally acquired island mediate Neisseria gonorrhoeae autolysis. by Katy Poncin, Samantha A McKeand, Hayley Lavender, Kacper Kurzyp, Odile B Harrison, Annabell Roberti, Charlotte Melia, Errin Johnson, Martin C J Maiden, David R Greaves, Rachel Exley, Christoph M Tang

“…Here, we searched for genes present in these 2 invasive species but not in other members of the Neisseria genus. …”
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Comprehensive landscape and oncogenic role of extrachromosomal circular DNA in malignant biliary strictures by Zhuo Cheng, Xuanmei Luo, Wenzheng Liu, Xiaofang Lu, Hong Chang, Yingchun Wang, Wei Zheng, Xiue Yan, Yonghui Huang

“…EccDNA carrying miR-106a/363 cluster or miR-374b/421 cluster were proven to regulate cancer gene expression, accelerate tumor proliferation, and inhibit tumor apoptosis. …”
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Integrating taxonomic, genetic and ecological data to explore the species richness of wild bees (Hymenoptera, Apoidea, Anthophila) of the Culuccia Peninsula (NE Sardinia, Italy) by Matteo Annessi, Alessandra Riccieri, Marilena Marconi, Sabrina Rossi, Andrea Di Giulio

“…DNA was extracted to amplify sequences of the mitochondrial gene Cyotochrome oxydase I, which were then compared with those in BOLD using the identification tool and by constructing neighbor-joining phylogenetic trees. …”
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Microbial community variations in adult Hyalomma dromedarii ticks from single locations in Saudi Arabia and Tunisia by Myriam Kratou, Apolline Maitre, Apolline Maitre, Apolline Maitre, Lianet Abuin-Denis, Lianet Abuin-Denis, Rachid Selmi, Hanène Belkahia, Abdullah D. Alanazi, Hattan Gattan, Hattan Gattan, Bassam M. Al-Ahmadi, Abdullah F. Shater, Lourdes Mateos-Hernández, Dasiel Obregón, Lilia Messadi, Alejandro Cabezas-Cruz, Mourad Ben Said, Mourad Ben Said

“…Despite prior studies on its microbiota, the effects of geographic origin and sex on microbial community structure and functional stability remain poorly understood.MethodsTo address this, we characterized the bacterial microbiota of H. dromedarii ticks collected from camels in Tunisia (TUN) and Saudi Arabia (SA) using 16S rRNA gene sequencing, microbial network analysis, and metabolic pathway prediction.ResultsOur findings indicate a dominant presence of Francisella endosymbionts in Tunisian ticks, suggesting adaptive roles of H. dromedarii ticks in arid ecosystems. …”
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Protein multiplicity: exemplifying an overwhelmingly likely pattern of molecular evolution? by Les Hatton, Greg Warr

“…We show that observed perturbations of the CoHSI-predicted distribution are strongly associated with enterobacterial species in which rapid microevolution facilitated by the horizontal transfer of genes is prevalent.…”
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Prevalence of resistance-associated substitutions (RAS) in hepatitis C virus in the Former Soviet Union countries by Sten H Vermund, Salima Davlidova, Syed Hani Abidi, Syed Ali, Robert Heimer, Aidana Mustafa, Dinara Begimbetova

“…This study’s objective was to observe the prevalence of HCV genotypes and RAS within the Former Soviet Union (FSU) countries.Methods We analysed 60 NS3, 313 NS5A and 1119 NS5B sequences of HCV deposited in open-access databases from 11 FSU countries for the prevalence of genotypes and the presence of RAS using the Geno2Pheno software.Results The following NS3 RASs were revealed through our analyses: 156P/S/T, 168del, 80K, 55A and 174S. …”
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Conditional similarity triplets enable covariate-informed representations of single-cell data by Chi-Jane Chen, Haidong Yi, Natalie Stanley

“…Abstract Background Single-cell technologies enable comprehensive profiling of diverse immune cell-types through the measurement of multiple genes or proteins per individual cell. In order to translate immune signatures assayed from blood or tissue into powerful diagnostics, machine learning approaches are often employed to compute immunological summaries or per-sample featurizations, which can be used as inputs to models for outcomes of interest. …”
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Digital twins as global learning health and disease models for preventive and personalized medicine by Xinxiu Li, Joseph Loscalzo, A. K. M. Firoj Mahmud, Dina Mansour Aly, Andrey Rzhetsky, Marinka Zitnik, Mikael Benson

“…This issue stems from the complex nature of diseases, which involve altered interactions among thousands of genes across multiple cell types and organs. Disease progression can vary between patients and over time, influenced by genetic and environmental factors. …”
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Differential expression and localization of expansins in Arabidopsis shoots: implications for cell wall dynamics and drought tolerance by Darina Balkova, Katerina Mala, Jan Hejatko, Jan Hejatko, Klara Panzarova, Lamis Abdelhakim, Barbora Pleskacova, Marketa Samalova, Marketa Samalova

“…Overexpression of EXPA1 resulted in pronounced changes in CW-related gene expression, particularly during early stages of induction, including the upregulation of other expansins and CW-modifying enzymes. …”
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Gut microbiota dysfunction in Crohn’s disease by Sylvie Buffet-Bataillon, Sylvie Buffet-Bataillon, Gabriela Durão, Isabelle Le Huërou-Luron, Olivier Rué, Olivier Rué, Yann Le Cunff, Vincent Cattoir, Guillaume Bouguen

“…Here, we investigated metagenomic functions using PICRUSt2 during the course of CD to better understand microbiota-related disease mechanisms and provide new insights for novel therapeutic strategies.MethodsWe performed 16S rRNA-based microbial profiling of 567 faecal samples collected from a cohort of 383 CD patients, including 291 remissions (CR), 177 mild-moderate (CM) and 99 severe (CS) disease states. Gene and pathway composition was assessed using PICRUSt2 analyses of 16S data.ResultsAs expected, changes in alpha and beta diversity, in interaction networks and increases in Proteobacteria abundance were associated with disease severity. …”
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Modulatory Impacts of Probiotic on the Synthesis of Claudins and Mucins in the Gastrointestinal Tract of Broiler Chicks by ESI Mohammed, M Al-Rasheed, MS Ahmed

“…Proventriculi, ilea, ceca, and cola samples were collected at days 0, 7, and 14 (D0, D7 and D14) for gene expression analysis. Results revealed that CLD1 expression was lower in probiotic groups at D0 and D14 in the proventriculus and colon, respectively, yet higher at D7 in the ileum and colon. …”
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