Retention of locally injected human iPS cell-derived cardiomyocytes into the myocardium using hydrolyzed gelatin
Abstract This study explored the impact of hydrolyzed gelatin (HG) concentration on the retention and therapeutic efficacy of human iPS cell-derived cardiomyocytes (hiPSC-CMs) when injected into the myocardium. The solubility of HG allows precise control over its concentration, influencing the distr...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-02-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-025-87885-w |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823862456524472320 |
|---|---|
| author | Jun Iida Kazuki Kotani Kozue Murata Keisuke Hakamada Wusiman Maihemuti Yoshinobu Mandai Yosuke Hiraoka Kenji Minatoya Hidetoshi Masumoto |
| author_facet | Jun Iida Kazuki Kotani Kozue Murata Keisuke Hakamada Wusiman Maihemuti Yoshinobu Mandai Yosuke Hiraoka Kenji Minatoya Hidetoshi Masumoto |
| author_sort | Jun Iida |
| collection | DOAJ |
| description | Abstract This study explored the impact of hydrolyzed gelatin (HG) concentration on the retention and therapeutic efficacy of human iPS cell-derived cardiomyocytes (hiPSC-CMs) when injected into the myocardium. The solubility of HG allows precise control over its concentration, influencing the distribution and leakage of injected solutions, which may affect therapeutic outcomes. Using both ex vivo and in vivo rat models, we investigated how varying HG concentrations affect the retention of solution and diffusion within the myocardium. In ex vivo static rat hearts, 10% HG minimized leakage but allowed significant diffusion. However, in pulsating in vivo hearts, 20% HG provided the best retention. In a rat myocardial infarction model, hiPSC-CMs suspended in 20% HG resulted in the highest cell retention. Echocardiogram showed a significant increase in the ejection fraction two weeks after transplantation compared to before transplantation. Additionally, cardiac magnetic resonance imaging (MRI) revealed that the ejection fraction was significantly higher than that of the sham group four weeks after transplantation. These findings suggest that optimizing HG concentration is crucial for enhancing the retention and therapeutic efficacy of hiPSC-CM transplants in treating heart disease. |
| format | Article |
| id | doaj-art-13f389a25a0d4223b548544163a52a15 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-13f389a25a0d4223b548544163a52a152025-02-09T12:31:55ZengNature PortfolioScientific Reports2045-23222025-02-0115111110.1038/s41598-025-87885-wRetention of locally injected human iPS cell-derived cardiomyocytes into the myocardium using hydrolyzed gelatinJun Iida0Kazuki Kotani1Kozue Murata2Keisuke Hakamada3Wusiman Maihemuti4Yoshinobu Mandai5Yosuke Hiraoka6Kenji Minatoya7Hidetoshi Masumoto8Clinical Translational Research Program, RIKEN Center for Biosystems Dynamics ResearchBiomedical Department, R&D Center, Nitta Gelatin IncClinical Translational Research Program, RIKEN Center for Biosystems Dynamics ResearchDepartment of Cardiovascular Surgery, Graduate School of Medicine, Kyoto UniversityClinical Translational Research Program, RIKEN Center for Biosystems Dynamics ResearchBiomedical Department, R&D Center, Nitta Gelatin IncBiomedical Department, R&D Center, Nitta Gelatin IncDepartment of Cardiovascular Surgery, Graduate School of Medicine, Kyoto UniversityClinical Translational Research Program, RIKEN Center for Biosystems Dynamics ResearchAbstract This study explored the impact of hydrolyzed gelatin (HG) concentration on the retention and therapeutic efficacy of human iPS cell-derived cardiomyocytes (hiPSC-CMs) when injected into the myocardium. The solubility of HG allows precise control over its concentration, influencing the distribution and leakage of injected solutions, which may affect therapeutic outcomes. Using both ex vivo and in vivo rat models, we investigated how varying HG concentrations affect the retention of solution and diffusion within the myocardium. In ex vivo static rat hearts, 10% HG minimized leakage but allowed significant diffusion. However, in pulsating in vivo hearts, 20% HG provided the best retention. In a rat myocardial infarction model, hiPSC-CMs suspended in 20% HG resulted in the highest cell retention. Echocardiogram showed a significant increase in the ejection fraction two weeks after transplantation compared to before transplantation. Additionally, cardiac magnetic resonance imaging (MRI) revealed that the ejection fraction was significantly higher than that of the sham group four weeks after transplantation. These findings suggest that optimizing HG concentration is crucial for enhancing the retention and therapeutic efficacy of hiPSC-CM transplants in treating heart disease.https://doi.org/10.1038/s41598-025-87885-w |
| spellingShingle | Jun Iida Kazuki Kotani Kozue Murata Keisuke Hakamada Wusiman Maihemuti Yoshinobu Mandai Yosuke Hiraoka Kenji Minatoya Hidetoshi Masumoto Retention of locally injected human iPS cell-derived cardiomyocytes into the myocardium using hydrolyzed gelatin Scientific Reports |
| title | Retention of locally injected human iPS cell-derived cardiomyocytes into the myocardium using hydrolyzed gelatin |
| title_full | Retention of locally injected human iPS cell-derived cardiomyocytes into the myocardium using hydrolyzed gelatin |
| title_fullStr | Retention of locally injected human iPS cell-derived cardiomyocytes into the myocardium using hydrolyzed gelatin |
| title_full_unstemmed | Retention of locally injected human iPS cell-derived cardiomyocytes into the myocardium using hydrolyzed gelatin |
| title_short | Retention of locally injected human iPS cell-derived cardiomyocytes into the myocardium using hydrolyzed gelatin |
| title_sort | retention of locally injected human ips cell derived cardiomyocytes into the myocardium using hydrolyzed gelatin |
| url | https://doi.org/10.1038/s41598-025-87885-w |
| work_keys_str_mv | AT juniida retentionoflocallyinjectedhumanipscellderivedcardiomyocytesintothemyocardiumusinghydrolyzedgelatin AT kazukikotani retentionoflocallyinjectedhumanipscellderivedcardiomyocytesintothemyocardiumusinghydrolyzedgelatin AT kozuemurata retentionoflocallyinjectedhumanipscellderivedcardiomyocytesintothemyocardiumusinghydrolyzedgelatin AT keisukehakamada retentionoflocallyinjectedhumanipscellderivedcardiomyocytesintothemyocardiumusinghydrolyzedgelatin AT wusimanmaihemuti retentionoflocallyinjectedhumanipscellderivedcardiomyocytesintothemyocardiumusinghydrolyzedgelatin AT yoshinobumandai retentionoflocallyinjectedhumanipscellderivedcardiomyocytesintothemyocardiumusinghydrolyzedgelatin AT yosukehiraoka retentionoflocallyinjectedhumanipscellderivedcardiomyocytesintothemyocardiumusinghydrolyzedgelatin AT kenjiminatoya retentionoflocallyinjectedhumanipscellderivedcardiomyocytesintothemyocardiumusinghydrolyzedgelatin AT hidetoshimasumoto retentionoflocallyinjectedhumanipscellderivedcardiomyocytesintothemyocardiumusinghydrolyzedgelatin |