ANALYSIS OF IMMUNITY IN OSTEOSARCOMA AS A BASIS FOR DEVELOPMENT FOLLOWING THERAPY
Background: Osteosarcoma is a malignant bone disease. Predictors which used to determine osteosarcoma from non-invasive (stage IIB) to invasive (stage III), through the ratio of macrophages-1 (M-1) which are pro-inflammatory (anti-cancer) with macrophages-2 (M-2) which have anti-inflammatory charact...
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| Format: | Article |
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Universitas Airlangga
2019-12-01
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| Series: | Journal Orthopaedi and Traumatology Surabaya |
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| Online Access: | https://e-journal.unair.ac.id/JOINTS/article/view/16571 |
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| author | Ferdiansyah Mahyudin Mouli Edward Muhammad Hardian Basuki Kadek Seta Prawira |
| author_facet | Ferdiansyah Mahyudin Mouli Edward Muhammad Hardian Basuki Kadek Seta Prawira |
| author_sort | Ferdiansyah Mahyudin |
| collection | DOAJ |
| description | Background: Osteosarcoma is a malignant bone disease. Predictors which used to determine osteosarcoma from non-invasive (stage IIB) to invasive (stage III), through the ratio of macrophages-1 (M-1) which are pro-inflammatory (anti-cancer) with macrophages-2 (M-2) which have anti-inflammatory characteristic (pro cancer) is not clear yet.
Purpose: To elaborate the role of M-1 ratio with M-2 in the development of non-invasive osteosarcoma become invasive, in effort to obtain predictors that can be used to the selection of neoadjuvant therapy time.
Methods: An observational study with cross sectional analytic study design. The sample of this research were all of paraffin blocks containing osteosarcoma patient tissue, collected within 5 years. The number of samples that met the inclusion criteria was 26 samples, and then divided into two groups, namely group-I as many as 13 stages IIB and group-II as many as 13 samples of stage III. The measured variables are the number of macrophages that express TNF-α and those that express IL-10 by immunohistochemical methods.
Results: Analysis of the data using a Mann-Whitney test, and the results were obtain is the number of macrophages expressing TNF-α in group-I was greater than in group-II (p <0.05). Likewise, macrophages that expressed IL-10 in group I were smaller than in group-II (p <0.05).
Conclusion: The M-1 ratio which reflected by TNF-α expression cells with M-2 as reflected by IL-10 expression cells in the non-invasive group is 5: 1.Whereas in the group that develops to invasive is 1:6. |
| format | Article |
| id | doaj-art-2637d8b325fc46f793283b2ccde15472 |
| institution | Kabale University |
| issn | 2722-712X 2460-8742 |
| language | English |
| publishDate | 2019-12-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Journal Orthopaedi and Traumatology Surabaya |
| spelling | doaj-art-2637d8b325fc46f793283b2ccde154722025-02-12T07:29:22ZengUniversitas AirlanggaJournal Orthopaedi and Traumatology Surabaya2722-712X2460-87422019-12-017213914810.20473/joints.v7i2.2018.139-14813521ANALYSIS OF IMMUNITY IN OSTEOSARCOMA AS A BASIS FOR DEVELOPMENT FOLLOWING THERAPYFerdiansyah Mahyudin0Mouli Edward1Muhammad Hardian Basuki2Kadek Seta Prawira3Staff of Orthopaedic and Traumatology Department, Faculty of Medicine, Universitas Airlangga, Dr Soetomo General Hospital, SurabayaStaff of Orthopaedic and Traumatology Department, Faculty of Medicine, Universitas Airlangga, Dr Soetomo General Hospital, SurabayaStaff of Orthopaedic and Traumatology Department, Faculty of Medicine, Universitas Airlangga, Dr Soetomo General Hospital, SurabayaResident of Orthopaedic and Traumatology Department, Faculty of Medicine, Universitas Airlangga, Dr Soetomo General Hospital, SurabayaBackground: Osteosarcoma is a malignant bone disease. Predictors which used to determine osteosarcoma from non-invasive (stage IIB) to invasive (stage III), through the ratio of macrophages-1 (M-1) which are pro-inflammatory (anti-cancer) with macrophages-2 (M-2) which have anti-inflammatory characteristic (pro cancer) is not clear yet. Purpose: To elaborate the role of M-1 ratio with M-2 in the development of non-invasive osteosarcoma become invasive, in effort to obtain predictors that can be used to the selection of neoadjuvant therapy time. Methods: An observational study with cross sectional analytic study design. The sample of this research were all of paraffin blocks containing osteosarcoma patient tissue, collected within 5 years. The number of samples that met the inclusion criteria was 26 samples, and then divided into two groups, namely group-I as many as 13 stages IIB and group-II as many as 13 samples of stage III. The measured variables are the number of macrophages that express TNF-α and those that express IL-10 by immunohistochemical methods. Results: Analysis of the data using a Mann-Whitney test, and the results were obtain is the number of macrophages expressing TNF-α in group-I was greater than in group-II (p <0.05). Likewise, macrophages that expressed IL-10 in group I were smaller than in group-II (p <0.05). Conclusion: The M-1 ratio which reflected by TNF-α expression cells with M-2 as reflected by IL-10 expression cells in the non-invasive group is 5: 1.Whereas in the group that develops to invasive is 1:6.https://e-journal.unair.ac.id/JOINTS/article/view/16571osteosarcomainflammationm-1- m-2 polarizationmetastasis |
| spellingShingle | Ferdiansyah Mahyudin Mouli Edward Muhammad Hardian Basuki Kadek Seta Prawira ANALYSIS OF IMMUNITY IN OSTEOSARCOMA AS A BASIS FOR DEVELOPMENT FOLLOWING THERAPY Journal Orthopaedi and Traumatology Surabaya osteosarcoma inflammation m-1- m-2 polarization metastasis |
| title | ANALYSIS OF IMMUNITY IN OSTEOSARCOMA AS A BASIS FOR DEVELOPMENT FOLLOWING THERAPY |
| title_full | ANALYSIS OF IMMUNITY IN OSTEOSARCOMA AS A BASIS FOR DEVELOPMENT FOLLOWING THERAPY |
| title_fullStr | ANALYSIS OF IMMUNITY IN OSTEOSARCOMA AS A BASIS FOR DEVELOPMENT FOLLOWING THERAPY |
| title_full_unstemmed | ANALYSIS OF IMMUNITY IN OSTEOSARCOMA AS A BASIS FOR DEVELOPMENT FOLLOWING THERAPY |
| title_short | ANALYSIS OF IMMUNITY IN OSTEOSARCOMA AS A BASIS FOR DEVELOPMENT FOLLOWING THERAPY |
| title_sort | analysis of immunity in osteosarcoma as a basis for development following therapy |
| topic | osteosarcoma inflammation m-1- m-2 polarization metastasis |
| url | https://e-journal.unair.ac.id/JOINTS/article/view/16571 |
| work_keys_str_mv | AT ferdiansyahmahyudin analysisofimmunityinosteosarcomaasabasisfordevelopmentfollowingtherapy AT mouliedward analysisofimmunityinosteosarcomaasabasisfordevelopmentfollowingtherapy AT muhammadhardianbasuki analysisofimmunityinosteosarcomaasabasisfordevelopmentfollowingtherapy AT kadeksetaprawira analysisofimmunityinosteosarcomaasabasisfordevelopmentfollowingtherapy |