Protocol to Retrieve Unknown Flanking DNA Using Fork PCR for Genome Walking

PCR-based genome walking is one of the prevalent techniques implemented to acquire unknown flanking genomic DNAs. The worth of genome walking includes but is not limited to cloning full-length genes, mining new genes, and discovering regulatory regions of genes. Therefore, this technique has advance...

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Bibliographic Details
Main Authors: Hongjing Wu, Hao Pan, Haixing Li
Format: Article
Language:English
Published: Bio-protocol LLC 2025-01-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=5161&type=0
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