Optimized production and antiviral activity evaluation of recombinant chicken interferon-alpha using an improved Baculovirus expression system
Despite extensive vaccination efforts, certain pathogens continue to pose significant challenges to the poultry industry. Interferons (IFNs) are crucial for innate immune defense against various pathogens. Chicken interferon-alpha (chIFN-α) reportedly has good inhibitory activity against various pat...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125001075 |
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| author | Yuxia Zhang Wenwen Dong Feng Li Kai Meng Guiming Li Xiaoyuan Yuan |
| author_facet | Yuxia Zhang Wenwen Dong Feng Li Kai Meng Guiming Li Xiaoyuan Yuan |
| author_sort | Yuxia Zhang |
| collection | DOAJ |
| description | Despite extensive vaccination efforts, certain pathogens continue to pose significant challenges to the poultry industry. Interferons (IFNs) are crucial for innate immune defense against various pathogens. Chicken interferon-alpha (chIFN-α) reportedly has good inhibitory activity against various pathogens. However, its production is often limited by low yields. This study aimed to enhance the production of chIFN-α using an improved baculovirus expression system and evaluate its antiviral efficacy both in vivo and in vitro. A recombinant bacmid carrying two copies of the chIFN-α gene was successfully constructed. The chIFN-α protein was obtained using the modified baculovirus expression system, and exhibited anti-vesicular stomatitis virus (VSV) activity of up to 5.0 × 106 IU/ml. Moreover, the administration of chIFN-α to SPF chicken inhibited H9N2 subtype low pathogenicity avian influenza virus (LPAIV) proliferation and reduced the viral-shedding rate. In the present study, we successfully expressed and purified chIFN-α at high yields, as well as demonstrated its strong antiviral activity in vivo and in vitro. These findings present an alternative approach for the efficient production of chIFN-α for therapeutic applications. |
| format | Article |
| id | doaj-art-8c1ff1bb46354219881db520dc191b66 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-8c1ff1bb46354219881db520dc191b662025-02-09T04:59:34ZengElsevierPoultry Science0032-57912025-03-011043104870Optimized production and antiviral activity evaluation of recombinant chicken interferon-alpha using an improved Baculovirus expression systemYuxia Zhang0Wenwen Dong1Feng Li2Kai Meng3Guiming Li4Xiaoyuan Yuan5Poultry Institute, Shandong Academy of Agricultural Sciences, Jinan Key Laboratory for Agricultural Experimental Animal and Comparative Medicine, Jinan 250100, ChinaPoultry Institute, Shandong Academy of Agricultural Sciences, Jinan Key Laboratory for Agricultural Experimental Animal and Comparative Medicine, Jinan 250100, ChinaPoultry Institute, Shandong Academy of Agricultural Sciences, Jinan Key Laboratory for Agricultural Experimental Animal and Comparative Medicine, Jinan 250100, ChinaPoultry Institute, Shandong Academy of Agricultural Sciences, Jinan Key Laboratory for Agricultural Experimental Animal and Comparative Medicine, Jinan 250100, ChinaPoultry Institute, Shandong Academy of Agricultural Sciences, Jinan Key Laboratory for Agricultural Experimental Animal and Comparative Medicine, Jinan 250100, ChinaCorresponding author.; Poultry Institute, Shandong Academy of Agricultural Sciences, Jinan Key Laboratory for Agricultural Experimental Animal and Comparative Medicine, Jinan 250100, ChinaDespite extensive vaccination efforts, certain pathogens continue to pose significant challenges to the poultry industry. Interferons (IFNs) are crucial for innate immune defense against various pathogens. Chicken interferon-alpha (chIFN-α) reportedly has good inhibitory activity against various pathogens. However, its production is often limited by low yields. This study aimed to enhance the production of chIFN-α using an improved baculovirus expression system and evaluate its antiviral efficacy both in vivo and in vitro. A recombinant bacmid carrying two copies of the chIFN-α gene was successfully constructed. The chIFN-α protein was obtained using the modified baculovirus expression system, and exhibited anti-vesicular stomatitis virus (VSV) activity of up to 5.0 × 106 IU/ml. Moreover, the administration of chIFN-α to SPF chicken inhibited H9N2 subtype low pathogenicity avian influenza virus (LPAIV) proliferation and reduced the viral-shedding rate. In the present study, we successfully expressed and purified chIFN-α at high yields, as well as demonstrated its strong antiviral activity in vivo and in vitro. These findings present an alternative approach for the efficient production of chIFN-α for therapeutic applications.http://www.sciencedirect.com/science/article/pii/S0032579125001075Chicken IFN-αBaculovirus expression systemRecombinant bacmidAntiviral activity |
| spellingShingle | Yuxia Zhang Wenwen Dong Feng Li Kai Meng Guiming Li Xiaoyuan Yuan Optimized production and antiviral activity evaluation of recombinant chicken interferon-alpha using an improved Baculovirus expression system Poultry Science Chicken IFN-α Baculovirus expression system Recombinant bacmid Antiviral activity |
| title | Optimized production and antiviral activity evaluation of recombinant chicken interferon-alpha using an improved Baculovirus expression system |
| title_full | Optimized production and antiviral activity evaluation of recombinant chicken interferon-alpha using an improved Baculovirus expression system |
| title_fullStr | Optimized production and antiviral activity evaluation of recombinant chicken interferon-alpha using an improved Baculovirus expression system |
| title_full_unstemmed | Optimized production and antiviral activity evaluation of recombinant chicken interferon-alpha using an improved Baculovirus expression system |
| title_short | Optimized production and antiviral activity evaluation of recombinant chicken interferon-alpha using an improved Baculovirus expression system |
| title_sort | optimized production and antiviral activity evaluation of recombinant chicken interferon alpha using an improved baculovirus expression system |
| topic | Chicken IFN-α Baculovirus expression system Recombinant bacmid Antiviral activity |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125001075 |
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