Microbial partner (MiPner) analysis
IntroductionAlthough a few bacteria have been studied in great depth, relatively little is known about the characteristics of microbe-microbe interactions that occur within ecosystems on a daily basis. A simple, robust technique was developed to set up the foundation for investigating pairwise bacte...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Microbiomes |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/frmbi.2024.1500798/full |
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| author | Jeffrey L. Bennetzen Josue Fernandez-Canela Vienna Elmgreen Shaugnessy R. McCann Mary E. Norris Xiangyu Deng Philip Brailey-Crane |
| author_facet | Jeffrey L. Bennetzen Josue Fernandez-Canela Vienna Elmgreen Shaugnessy R. McCann Mary E. Norris Xiangyu Deng Philip Brailey-Crane |
| author_sort | Jeffrey L. Bennetzen |
| collection | DOAJ |
| description | IntroductionAlthough a few bacteria have been studied in great depth, relatively little is known about the characteristics of microbe-microbe interactions that occur within ecosystems on a daily basis. A simple, robust technique was developed to set up the foundation for investigating pairwise bacterial-bacterial interactions, using cell-cell binding as a self-selective mechanism to identify interesting bacterial species pairs.MethodsUsing a Serratia marcescens strain (SMC43) isolated from Georgia soil as a “bait”, specific bacteria were purified by their specificity in binding SMC43 bacteria that were themselves attached to a wooden applicator stick.ResultsThe isolated Microbial Partners (MiPners) were greatly enriched for members of the genera Sphingobium and Caulobacter. Two streaked MiPners were unable to grow on the plates employed after separation from SMC43to be separated from, and grow on the plate type tested without, SMC43.DiscussionThis suggests that the MiPner technology will be one strategy for purifying bacteria that were previously recalcitrant to culturing. |
| format | Article |
| id | doaj-art-bb6e02d8d35f4fcf88382f778acb1de1 |
| institution | Kabale University |
| issn | 2813-4338 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiomes |
| spelling | doaj-art-bb6e02d8d35f4fcf88382f778acb1de12025-02-07T06:49:30ZengFrontiers Media S.A.Frontiers in Microbiomes2813-43382025-02-01310.3389/frmbi.2024.15007981500798Microbial partner (MiPner) analysisJeffrey L. Bennetzen0Josue Fernandez-Canela1Vienna Elmgreen2Shaugnessy R. McCann3Mary E. Norris4Xiangyu Deng5Philip Brailey-Crane6Department of Genetics, University of Georgia, Athens, GA, United StatesDepartment of Plant Biology, University of Georgia, Athens, GA, United StatesDepartment of Genetics, University of Georgia, Athens, GA, United StatesDepartment of Genetics, University of Georgia, Athens, GA, United StatesDepartment of Genetics, University of Georgia, Athens, GA, United StatesCenter for Food Safety, University of Georgia, Griffin, GA, United StatesDepartment of Genetics, University of Georgia, Athens, GA, United StatesIntroductionAlthough a few bacteria have been studied in great depth, relatively little is known about the characteristics of microbe-microbe interactions that occur within ecosystems on a daily basis. A simple, robust technique was developed to set up the foundation for investigating pairwise bacterial-bacterial interactions, using cell-cell binding as a self-selective mechanism to identify interesting bacterial species pairs.MethodsUsing a Serratia marcescens strain (SMC43) isolated from Georgia soil as a “bait”, specific bacteria were purified by their specificity in binding SMC43 bacteria that were themselves attached to a wooden applicator stick.ResultsThe isolated Microbial Partners (MiPners) were greatly enriched for members of the genera Sphingobium and Caulobacter. Two streaked MiPners were unable to grow on the plates employed after separation from SMC43to be separated from, and grow on the plate type tested without, SMC43.DiscussionThis suggests that the MiPner technology will be one strategy for purifying bacteria that were previously recalcitrant to culturing.https://www.frontiersin.org/articles/10.3389/frmbi.2024.1500798/fullmicrobiologyMiPnermicrobe-microbe bindingmicrobial genomicssyncom |
| spellingShingle | Jeffrey L. Bennetzen Josue Fernandez-Canela Vienna Elmgreen Shaugnessy R. McCann Mary E. Norris Xiangyu Deng Philip Brailey-Crane Microbial partner (MiPner) analysis Frontiers in Microbiomes microbiology MiPner microbe-microbe binding microbial genomics syncom |
| title | Microbial partner (MiPner) analysis |
| title_full | Microbial partner (MiPner) analysis |
| title_fullStr | Microbial partner (MiPner) analysis |
| title_full_unstemmed | Microbial partner (MiPner) analysis |
| title_short | Microbial partner (MiPner) analysis |
| title_sort | microbial partner mipner analysis |
| topic | microbiology MiPner microbe-microbe binding microbial genomics syncom |
| url | https://www.frontiersin.org/articles/10.3389/frmbi.2024.1500798/full |
| work_keys_str_mv | AT jeffreylbennetzen microbialpartnermipneranalysis AT josuefernandezcanela microbialpartnermipneranalysis AT viennaelmgreen microbialpartnermipneranalysis AT shaugnessyrmccann microbialpartnermipneranalysis AT maryenorris microbialpartnermipneranalysis AT xiangyudeng microbialpartnermipneranalysis AT philipbraileycrane microbialpartnermipneranalysis |