Exosome-like nanovesicles derived from kale juice enhance collagen production by downregulating Smad7 in human skin fibroblasts
Plant-derived exosome-like nanovesicles (ELNs) are critical mediators of cross-kingdom communication, modulating gene expression in animal cells despite their plant origin. In this study, we investigated the effects of glucoraphanin-enriched kale (GEK)-derived ELNs (GELNs) on collagen production in...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2025.1486572/full |
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| author | Peihan Hsu Yuriko Kamijyo Emiri Koike Saki Ichikawa Yifeng Zheng Tomohiro Ohno Shigeru Katayama Shigeru Katayama |
| author_facet | Peihan Hsu Yuriko Kamijyo Emiri Koike Saki Ichikawa Yifeng Zheng Tomohiro Ohno Shigeru Katayama Shigeru Katayama |
| author_sort | Peihan Hsu |
| collection | DOAJ |
| description | Plant-derived exosome-like nanovesicles (ELNs) are critical mediators of cross-kingdom communication, modulating gene expression in animal cells despite their plant origin. In this study, we investigated the effects of glucoraphanin-enriched kale (GEK)-derived ELNs (GELNs) on collagen production in normal human dermal fibroblasts NB1RGB. The ELNs isolated from GEK juice powder had particle sizes similar to those of typical exosomes. GELNs increased type I collagen expression in NB1RGB cells significantly. Microarray analysis demonstrated that GELN-derived total RNA upregulated the expression of genes related to extracellular matrix formation, including those involved in collagen synthesis. Further investigation revealed that microRNA-enriched fraction of GELNs promoted collagen production by inhibiting the expression of Smad7. These findings suggest that GELNs and their microRNA content enhance collagen production through the downregulation of Smad7. |
| format | Article |
| id | doaj-art-c287d1507b18426eba821007df2070bc |
| institution | Kabale University |
| issn | 2296-861X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj-art-c287d1507b18426eba821007df2070bc2025-02-10T05:16:05ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2025-02-011210.3389/fnut.2025.14865721486572Exosome-like nanovesicles derived from kale juice enhance collagen production by downregulating Smad7 in human skin fibroblastsPeihan Hsu0Yuriko Kamijyo1Emiri Koike2Saki Ichikawa3Yifeng Zheng4Tomohiro Ohno5Shigeru Katayama6Shigeru Katayama7Department of Agriculture, Graduate School of Science and Technology, Shinshu University, Nagano, JapanDepartment of Agriculture, Graduate School of Science and Technology, Shinshu University, Nagano, JapanDepartment of Agriculture, Graduate School of Science and Technology, Shinshu University, Nagano, JapanDepartment of Agriculture, Graduate School of Science and Technology, Shinshu University, Nagano, JapanInstitute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, Nagano, JapanYakult Health Foods Co., Ltd., Oita, JapanDepartment of Agriculture, Graduate School of Science and Technology, Shinshu University, Nagano, JapanInstitute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, Nagano, JapanPlant-derived exosome-like nanovesicles (ELNs) are critical mediators of cross-kingdom communication, modulating gene expression in animal cells despite their plant origin. In this study, we investigated the effects of glucoraphanin-enriched kale (GEK)-derived ELNs (GELNs) on collagen production in normal human dermal fibroblasts NB1RGB. The ELNs isolated from GEK juice powder had particle sizes similar to those of typical exosomes. GELNs increased type I collagen expression in NB1RGB cells significantly. Microarray analysis demonstrated that GELN-derived total RNA upregulated the expression of genes related to extracellular matrix formation, including those involved in collagen synthesis. Further investigation revealed that microRNA-enriched fraction of GELNs promoted collagen production by inhibiting the expression of Smad7. These findings suggest that GELNs and their microRNA content enhance collagen production through the downregulation of Smad7.https://www.frontiersin.org/articles/10.3389/fnut.2025.1486572/fullcollagenexosomesexosome-like nanovesiclesfibroblastskalemiRNA |
| spellingShingle | Peihan Hsu Yuriko Kamijyo Emiri Koike Saki Ichikawa Yifeng Zheng Tomohiro Ohno Shigeru Katayama Shigeru Katayama Exosome-like nanovesicles derived from kale juice enhance collagen production by downregulating Smad7 in human skin fibroblasts Frontiers in Nutrition collagen exosomes exosome-like nanovesicles fibroblasts kale miRNA |
| title | Exosome-like nanovesicles derived from kale juice enhance collagen production by downregulating Smad7 in human skin fibroblasts |
| title_full | Exosome-like nanovesicles derived from kale juice enhance collagen production by downregulating Smad7 in human skin fibroblasts |
| title_fullStr | Exosome-like nanovesicles derived from kale juice enhance collagen production by downregulating Smad7 in human skin fibroblasts |
| title_full_unstemmed | Exosome-like nanovesicles derived from kale juice enhance collagen production by downregulating Smad7 in human skin fibroblasts |
| title_short | Exosome-like nanovesicles derived from kale juice enhance collagen production by downregulating Smad7 in human skin fibroblasts |
| title_sort | exosome like nanovesicles derived from kale juice enhance collagen production by downregulating smad7 in human skin fibroblasts |
| topic | collagen exosomes exosome-like nanovesicles fibroblasts kale miRNA |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2025.1486572/full |
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