Astaxanthin relieves HT22 cells from LPS-induced inflammation and apoptosis by inhibiting oxygen species and regulating the TLR4/MyD88/NFκB signaling pathway
Astaxanthin exhibits promising anti-inflammatory effects in the treatment of neurodegenerative diseases. Our aim was to explore the mechanisms by which astaxanthin mitigates inflammation associated with these conditions. We treated HT22 cells with lipopolysaccharide (LPS), astaxanthin (ATX), and the...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464625000180 |
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| author | Tan Wang Rongrong Li Ping Niu Zhen Wei Donge Xie Huorong Huang Jiayao Pan Chunshu Rong |
| author_facet | Tan Wang Rongrong Li Ping Niu Zhen Wei Donge Xie Huorong Huang Jiayao Pan Chunshu Rong |
| author_sort | Tan Wang |
| collection | DOAJ |
| description | Astaxanthin exhibits promising anti-inflammatory effects in the treatment of neurodegenerative diseases. Our aim was to explore the mechanisms by which astaxanthin mitigates inflammation associated with these conditions. We treated HT22 cells with lipopolysaccharide (LPS), astaxanthin (ATX), and the TLR4 inhibitor TAK-242, employing CCK-8 assays, flow cytometry, and Western blotting to assess cell survival, oxidative stress, inflammation, and apoptosis. We found that LPS decreased the survival rate of HT22 cells, induced reactive oxygen species (ROS) accumulation and apoptosis, and increased the expression of inflammation-related proteins. Conversely, ATX diminished LPS-induced ROS accumulation and apoptosis, as well as the expression of inflammation-related proteins. The application of TLR4 inhibitors further enhanced the anti-inflammatory effects of ATX on LPS. In summary, we demonstrated that ATX can alleviate LPS-induced ROS accumulation, inflammation, and apoptosis, with its anti-inflammatory effects closely linked to the TLR4/MyD88/NFκB signaling pathway. |
| format | Article |
| id | doaj-art-ca970fd006f9405db7ea74a25032d197 |
| institution | Kabale University |
| issn | 1756-4646 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj-art-ca970fd006f9405db7ea74a25032d1972025-02-09T04:59:56ZengElsevierJournal of Functional Foods1756-46462025-02-01125106676Astaxanthin relieves HT22 cells from LPS-induced inflammation and apoptosis by inhibiting oxygen species and regulating the TLR4/MyD88/NFκB signaling pathwayTan Wang0Rongrong Li1Ping Niu2Zhen Wei3Donge Xie4Huorong Huang5Jiayao Pan6Chunshu Rong7Department of General Surgery, Lianshui People's Hospital of Kangda College Affiliated to Nanjing Medical University,Huai 'an City, Jiangsu Province, ChinaDepartment of Neurology, Huaian 82nd Hospital, Huai 'an City, Jiangsu Province, ChinaDepartment of Encephalopathy, Hospital of Changchun University of Chinese Medicine, Changchun, Jilin 130021, ChinaDepartment of emergency, Hospital of Changchun University of Chinese Medicine, Changchun, Jilin 130021, ChinaDepartment of acupuncture, Hospital of Changchun University of Chinese Medicine, Changchun, Jilin 130021, ChinaDepartment of Encephalopathy, Hospital of Changchun University of Chinese Medicine, Changchun, Jilin 130021, ChinaDepartment of Encephalopathy, Hospital of Changchun University of Chinese Medicine, Changchun, Jilin 130021, ChinaDepartment of Encephalopathy, Hospital of Changchun University of Chinese Medicine, Changchun, Jilin 130021, China; Corresponding author.Astaxanthin exhibits promising anti-inflammatory effects in the treatment of neurodegenerative diseases. Our aim was to explore the mechanisms by which astaxanthin mitigates inflammation associated with these conditions. We treated HT22 cells with lipopolysaccharide (LPS), astaxanthin (ATX), and the TLR4 inhibitor TAK-242, employing CCK-8 assays, flow cytometry, and Western blotting to assess cell survival, oxidative stress, inflammation, and apoptosis. We found that LPS decreased the survival rate of HT22 cells, induced reactive oxygen species (ROS) accumulation and apoptosis, and increased the expression of inflammation-related proteins. Conversely, ATX diminished LPS-induced ROS accumulation and apoptosis, as well as the expression of inflammation-related proteins. The application of TLR4 inhibitors further enhanced the anti-inflammatory effects of ATX on LPS. In summary, we demonstrated that ATX can alleviate LPS-induced ROS accumulation, inflammation, and apoptosis, with its anti-inflammatory effects closely linked to the TLR4/MyD88/NFκB signaling pathway.http://www.sciencedirect.com/science/article/pii/S1756464625000180Neurodegenerative disordersAstaxanthinInflammationApoptosisOxidative stress |
| spellingShingle | Tan Wang Rongrong Li Ping Niu Zhen Wei Donge Xie Huorong Huang Jiayao Pan Chunshu Rong Astaxanthin relieves HT22 cells from LPS-induced inflammation and apoptosis by inhibiting oxygen species and regulating the TLR4/MyD88/NFκB signaling pathway Journal of Functional Foods Neurodegenerative disorders Astaxanthin Inflammation Apoptosis Oxidative stress |
| title | Astaxanthin relieves HT22 cells from LPS-induced inflammation and apoptosis by inhibiting oxygen species and regulating the TLR4/MyD88/NFκB signaling pathway |
| title_full | Astaxanthin relieves HT22 cells from LPS-induced inflammation and apoptosis by inhibiting oxygen species and regulating the TLR4/MyD88/NFκB signaling pathway |
| title_fullStr | Astaxanthin relieves HT22 cells from LPS-induced inflammation and apoptosis by inhibiting oxygen species and regulating the TLR4/MyD88/NFκB signaling pathway |
| title_full_unstemmed | Astaxanthin relieves HT22 cells from LPS-induced inflammation and apoptosis by inhibiting oxygen species and regulating the TLR4/MyD88/NFκB signaling pathway |
| title_short | Astaxanthin relieves HT22 cells from LPS-induced inflammation and apoptosis by inhibiting oxygen species and regulating the TLR4/MyD88/NFκB signaling pathway |
| title_sort | astaxanthin relieves ht22 cells from lps induced inflammation and apoptosis by inhibiting oxygen species and regulating the tlr4 myd88 nfκb signaling pathway |
| topic | Neurodegenerative disorders Astaxanthin Inflammation Apoptosis Oxidative stress |
| url | http://www.sciencedirect.com/science/article/pii/S1756464625000180 |
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