Platelet-rich fibrin and silver nano-particles loaded chitosan treatment for post- laminectomy epidural scar adhesions: in vivo rats study model
Abstract Objective Epidural scar fibrosis commonly leads to functional disability and pain following spinal surgery and is a prevalent manifestation of Failed Back Surgery Syndrome (FBSS). This study aimed to evaluate the use of silver nano-articles (AgNPs) loaded on chitosan (Chi/Ag-NPs) with plate...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-02-01
|
| Series: | BMC Neuroscience |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12868-025-00929-9 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1825197678751907840 |
|---|---|
| author | Samah Fouad Awad Rizk Esam Mosbah Mostafa M. Nabeeh Walaa Awadin Ayman S. Elmezayyen Ekramy Elmorsy Adel Zaghloul |
| author_facet | Samah Fouad Awad Rizk Esam Mosbah Mostafa M. Nabeeh Walaa Awadin Ayman S. Elmezayyen Ekramy Elmorsy Adel Zaghloul |
| author_sort | Samah Fouad |
| collection | DOAJ |
| description | Abstract Objective Epidural scar fibrosis commonly leads to functional disability and pain following spinal surgery and is a prevalent manifestation of Failed Back Surgery Syndrome (FBSS). This study aimed to evaluate the use of silver nano-articles (AgNPs) loaded on chitosan (Chi/Ag-NPs) with platelets-rich fibrin (PRF) gel for the reduction of post-laminectomy epidural scar adhesions. Methods A total of 90 male Sprague Dawley rats (255 ± 55gm) were randomized in-to six groups, each group with 15 rats: control group, laminectomy group, PRF group, Chi/Ag-NPs group, combined treatment group (PRF + Chi/Ag-NPs), and a group to prepare PRF. Lumbar laminectomy procedures were performed between L3-L5 in all rats except the control group. After a 30-days follow-up, macroscopic examination, histological studies, and mRNA evaluation for TGFβ-1and IL-6, were conducted. Results Data revealed that epidural scar adhesion, scaring, arachnoid involvement, dural thickness, as well as inflammation and TGFβ-1and IL-6 coding genes expression were significantly reduced in PRF group, Chi/Ag-NPs group, and combined group compared to the laminectomy group. Combined treatment showed more significant better outcomes. Conclusion The use of PRF with Chi/Ag-NPs as nano biomaterials could be considered a combination therapy for the reduction of EF post-laminectomy in a rat model. |
| format | Article |
| id | doaj-art-d1721c6d4dd54239a66ca3a047a1fe10 |
| institution | Kabale University |
| issn | 1471-2202 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Neuroscience |
| spelling | doaj-art-d1721c6d4dd54239a66ca3a047a1fe102025-02-09T12:14:05ZengBMCBMC Neuroscience1471-22022025-02-0126111310.1186/s12868-025-00929-9Platelet-rich fibrin and silver nano-particles loaded chitosan treatment for post- laminectomy epidural scar adhesions: in vivo rats study modelSamah Fouad0Awad Rizk1Esam Mosbah2Mostafa M. Nabeeh3Walaa Awadin4Ayman S. Elmezayyen5Ekramy Elmorsy6Adel Zaghloul7Medical Experimental Research Center (MERC), Faculty of Medicine, Mansoura UniversityDepartment of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Mansoura UniversityDepartment of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Mansoura UniversityDepartment of Neurosurgery, Faculty of Medicine, Mansoura UniversityDepartment of Pathology, Faculty of Veterinary Medicine, Mansoura UniversityPhysics Department, Faculty of Science, Mansoura UniversityCenter for Health Research, Northern Border UniversityDepartment of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Mansoura UniversityAbstract Objective Epidural scar fibrosis commonly leads to functional disability and pain following spinal surgery and is a prevalent manifestation of Failed Back Surgery Syndrome (FBSS). This study aimed to evaluate the use of silver nano-articles (AgNPs) loaded on chitosan (Chi/Ag-NPs) with platelets-rich fibrin (PRF) gel for the reduction of post-laminectomy epidural scar adhesions. Methods A total of 90 male Sprague Dawley rats (255 ± 55gm) were randomized in-to six groups, each group with 15 rats: control group, laminectomy group, PRF group, Chi/Ag-NPs group, combined treatment group (PRF + Chi/Ag-NPs), and a group to prepare PRF. Lumbar laminectomy procedures were performed between L3-L5 in all rats except the control group. After a 30-days follow-up, macroscopic examination, histological studies, and mRNA evaluation for TGFβ-1and IL-6, were conducted. Results Data revealed that epidural scar adhesion, scaring, arachnoid involvement, dural thickness, as well as inflammation and TGFβ-1and IL-6 coding genes expression were significantly reduced in PRF group, Chi/Ag-NPs group, and combined group compared to the laminectomy group. Combined treatment showed more significant better outcomes. Conclusion The use of PRF with Chi/Ag-NPs as nano biomaterials could be considered a combination therapy for the reduction of EF post-laminectomy in a rat model.https://doi.org/10.1186/s12868-025-00929-9Epidural scar fibrosisLaminectomyPlatelets-rich fibrinSilver nanoparticle loaded chitosan |
| spellingShingle | Samah Fouad Awad Rizk Esam Mosbah Mostafa M. Nabeeh Walaa Awadin Ayman S. Elmezayyen Ekramy Elmorsy Adel Zaghloul Platelet-rich fibrin and silver nano-particles loaded chitosan treatment for post- laminectomy epidural scar adhesions: in vivo rats study model BMC Neuroscience Epidural scar fibrosis Laminectomy Platelets-rich fibrin Silver nanoparticle loaded chitosan |
| title | Platelet-rich fibrin and silver nano-particles loaded chitosan treatment for post- laminectomy epidural scar adhesions: in vivo rats study model |
| title_full | Platelet-rich fibrin and silver nano-particles loaded chitosan treatment for post- laminectomy epidural scar adhesions: in vivo rats study model |
| title_fullStr | Platelet-rich fibrin and silver nano-particles loaded chitosan treatment for post- laminectomy epidural scar adhesions: in vivo rats study model |
| title_full_unstemmed | Platelet-rich fibrin and silver nano-particles loaded chitosan treatment for post- laminectomy epidural scar adhesions: in vivo rats study model |
| title_short | Platelet-rich fibrin and silver nano-particles loaded chitosan treatment for post- laminectomy epidural scar adhesions: in vivo rats study model |
| title_sort | platelet rich fibrin and silver nano particles loaded chitosan treatment for post laminectomy epidural scar adhesions in vivo rats study model |
| topic | Epidural scar fibrosis Laminectomy Platelets-rich fibrin Silver nanoparticle loaded chitosan |
| url | https://doi.org/10.1186/s12868-025-00929-9 |
| work_keys_str_mv | AT samahfouad plateletrichfibrinandsilvernanoparticlesloadedchitosantreatmentforpostlaminectomyepiduralscaradhesionsinvivoratsstudymodel AT awadrizk plateletrichfibrinandsilvernanoparticlesloadedchitosantreatmentforpostlaminectomyepiduralscaradhesionsinvivoratsstudymodel AT esammosbah plateletrichfibrinandsilvernanoparticlesloadedchitosantreatmentforpostlaminectomyepiduralscaradhesionsinvivoratsstudymodel AT mostafamnabeeh plateletrichfibrinandsilvernanoparticlesloadedchitosantreatmentforpostlaminectomyepiduralscaradhesionsinvivoratsstudymodel AT walaaawadin plateletrichfibrinandsilvernanoparticlesloadedchitosantreatmentforpostlaminectomyepiduralscaradhesionsinvivoratsstudymodel AT aymanselmezayyen plateletrichfibrinandsilvernanoparticlesloadedchitosantreatmentforpostlaminectomyepiduralscaradhesionsinvivoratsstudymodel AT ekramyelmorsy plateletrichfibrinandsilvernanoparticlesloadedchitosantreatmentforpostlaminectomyepiduralscaradhesionsinvivoratsstudymodel AT adelzaghloul plateletrichfibrinandsilvernanoparticlesloadedchitosantreatmentforpostlaminectomyepiduralscaradhesionsinvivoratsstudymodel |