MicroRNA-541-3p/Rac2 signaling bridges radiation-induced lung injury and repair
Background: While radiation-induced lung injury decreases quality of life and suppresses efficacy of radiotherapy, to date, the relationship between radiation-induced lung injury and repair remains unclear. Our previous studies revealed that TNFRSF10B-RIPK1/RIPK3-MLKL signaling induces necroptosis o...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2025-06-01
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| Series: | Non-coding RNA Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468054025000228 |
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| author | Jiandong Zhang Lei Ma Limin He Quanxiao Xu Yan Ding Lidong Wang |
| author_facet | Jiandong Zhang Lei Ma Limin He Quanxiao Xu Yan Ding Lidong Wang |
| author_sort | Jiandong Zhang |
| collection | DOAJ |
| description | Background: While radiation-induced lung injury decreases quality of life and suppresses efficacy of radiotherapy, to date, the relationship between radiation-induced lung injury and repair remains unclear. Our previous studies revealed that TNFRSF10B-RIPK1/RIPK3-MLKL signaling induces necroptosis of alveolar epithelial cells and potentiates radiation-induced lung injury. We also found that microRNA-541-3p is differentially expressed in radiation-damaged lungs. The connection between microRNA-541-3p, TNFRSF10B signaling, and TGFβ1 signaling is also unclear. Objective: This study was performed to explore the regulatory effects of microRNA-541-3p on TNFRSF10B and TGFβ1 signaling. Methods: Mouse alveolar epithelial cells were transfected with a vector expressing microRNA-541-3p to regulate expression of target genes. Flow cytometry, polymerase chain reaction, and western blotting were used to analyze cell necroptosis, target gene expression, and target protein expression, respectively. Results: Overexpression of microRNA-541-3p positively regulated TNFRSF10B-RIPK1/RIPK3-MLKL signaling through Rac2 to induce cell necroptosis. MicroRNA-541-3p negatively regulates Rac2. MicroRNA-541-3p and Rac2 regulate the expression of Tgf-beta1 and its encoded proteins. Conclusions: The Rac2 gene synchronously regulates TNFRSF10B-RIPK1/RIPK3-MLKL and TGFβ1 signaling. MicroRNA-541-3P/Rac2 act as mediators of radiation damage and repair signaling. |
| format | Article |
| id | doaj-art-e2eff53042c547fca6375a8f5352bda0 |
| institution | Kabale University |
| issn | 2468-0540 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Non-coding RNA Research |
| spelling | doaj-art-e2eff53042c547fca6375a8f5352bda02025-02-12T05:31:30ZengKeAi Communications Co., Ltd.Non-coding RNA Research2468-05402025-06-01121019MicroRNA-541-3p/Rac2 signaling bridges radiation-induced lung injury and repairJiandong Zhang0Lei Ma1Limin He2Quanxiao Xu3Yan Ding4Lidong Wang5Clinical School of Medicine, Henan University of Science and Technology, Luoyang City, Henan Province, 471023, China; The First Affiliated Hospital of Henan University of Science and Technology, Luoyang City, Henan Province, 471023, ChinaDepartment of Radiation Oncology, Nanyang First People's Hospital Affiliated to Henan University, Nanyang City, Henan Province, 473000, ChinaDepartment of Radiation Oncology, Nanyang First People's Hospital Affiliated to Henan University, Nanyang City, Henan Province, 473000, ChinaDepartment of Oncology, the Second Affiliated Hospital of Soochow University, Suzhou City, Jiangsu Province, 215000, ChinaDepartment of Radiation Oncology, Nanyang First People's Hospital Affiliated to Henan University, Nanyang City, Henan Province, 473000, ChinaClinical School of Medicine, Henan University of Science and Technology, Luoyang City, Henan Province, 471023, China; The First Affiliated Hospital of Henan University of Science and Technology, Luoyang City, Henan Province, 471023, China; Corresponding author. No. 263, Kaiyuan Avenue, Luolong District, Clinical School of Medicine, Henan University of Science and Technology, Luoyang City, Henan Province, 471023, China.Background: While radiation-induced lung injury decreases quality of life and suppresses efficacy of radiotherapy, to date, the relationship between radiation-induced lung injury and repair remains unclear. Our previous studies revealed that TNFRSF10B-RIPK1/RIPK3-MLKL signaling induces necroptosis of alveolar epithelial cells and potentiates radiation-induced lung injury. We also found that microRNA-541-3p is differentially expressed in radiation-damaged lungs. The connection between microRNA-541-3p, TNFRSF10B signaling, and TGFβ1 signaling is also unclear. Objective: This study was performed to explore the regulatory effects of microRNA-541-3p on TNFRSF10B and TGFβ1 signaling. Methods: Mouse alveolar epithelial cells were transfected with a vector expressing microRNA-541-3p to regulate expression of target genes. Flow cytometry, polymerase chain reaction, and western blotting were used to analyze cell necroptosis, target gene expression, and target protein expression, respectively. Results: Overexpression of microRNA-541-3p positively regulated TNFRSF10B-RIPK1/RIPK3-MLKL signaling through Rac2 to induce cell necroptosis. MicroRNA-541-3p negatively regulates Rac2. MicroRNA-541-3p and Rac2 regulate the expression of Tgf-beta1 and its encoded proteins. Conclusions: The Rac2 gene synchronously regulates TNFRSF10B-RIPK1/RIPK3-MLKL and TGFβ1 signaling. MicroRNA-541-3P/Rac2 act as mediators of radiation damage and repair signaling.http://www.sciencedirect.com/science/article/pii/S2468054025000228MicroRNA-541-3pRac2TNFRSF10BTGFβ1Radiation-induced lung injury |
| spellingShingle | Jiandong Zhang Lei Ma Limin He Quanxiao Xu Yan Ding Lidong Wang MicroRNA-541-3p/Rac2 signaling bridges radiation-induced lung injury and repair Non-coding RNA Research MicroRNA-541-3p Rac2 TNFRSF10B TGFβ1 Radiation-induced lung injury |
| title | MicroRNA-541-3p/Rac2 signaling bridges radiation-induced lung injury and repair |
| title_full | MicroRNA-541-3p/Rac2 signaling bridges radiation-induced lung injury and repair |
| title_fullStr | MicroRNA-541-3p/Rac2 signaling bridges radiation-induced lung injury and repair |
| title_full_unstemmed | MicroRNA-541-3p/Rac2 signaling bridges radiation-induced lung injury and repair |
| title_short | MicroRNA-541-3p/Rac2 signaling bridges radiation-induced lung injury and repair |
| title_sort | microrna 541 3p rac2 signaling bridges radiation induced lung injury and repair |
| topic | MicroRNA-541-3p Rac2 TNFRSF10B TGFβ1 Radiation-induced lung injury |
| url | http://www.sciencedirect.com/science/article/pii/S2468054025000228 |
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