Recombinant AAV batch profiling by nanopore sequencing elucidates product-related DNA impurities and vector genome length distribution

Recombinant AAV batch profiling by nanopore sequencing elucidates product-related DNA impurities and vector genome length distribution

During production, recombinant adeno-associated virus (rAAV) capsids are equipped with heterogeneous genetic payloads including undesired DNA impurities as well as truncated vector genomes. Comprehensive analysis of encapsidated DNA by long-read next-generation sequencing is destined to guide platfo...

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Bibliographic Details
Main Authors: Florian Dunker-Seidler, Kathrin Breunig, Magdalena Haubner, Florian Sonntag, Markus Hörer, Rebecca C. Feiner
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:Molecular Therapy: Methods & Clinical Development
Subjects:
nanopore sequencing, gene therapy
adeno-associated virus, AAV
analytics
bioinformatics
Online Access:http://www.sciencedirect.com/science/article/pii/S2329050125000129
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