CRISPR-Cas9-based one-step multiplexed genome editing through optimizing guide RNA processing strategies in Pichia pastoris

CRISPR-Cas9-based one-step multiplexed genome editing through optimizing guide RNA processing strategies in Pichia pastoris

The important methylotrophic yeast Pichia pastoris has been utilized for the production of a variety of heterologous recombinant proteins and has great potential for use in the production of value-added compounds using methanol as a substrate. However, the lack of convenient and efficient genome eng...

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Bibliographic Details
Main Authors: Kaidi Chen, Gulikezi Maimaitirexiati, Qiannan Zhang, Yi Li, Xiangjian Liu, Hongting Tang, Xiang Gao, Bo Wang, Tao Yu, Shuyuan Guo
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2025-06-01
Series:Synthetic and Systems Biotechnology
Subjects:
Pichia pastoris
Gene deletion and integration
CRISPR/Cas9
Genome editing
gRNA processing
Online Access:http://www.sciencedirect.com/science/article/pii/S2405805X25000055
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