Assessing the probiotic potential of Lactococcus lactis isolated from the intestine and gill of rainbow trout (Oncorhynchus mykiss) through in vitro analysis
The emergence of diseases and the indiscriminate use of antibiotics have had deleterious consequences in the aquaculture industry. Consequently, the growing emphasis on eco-friendly alternatives has taken center stage, and probiotics have emerged as a notable solution. This investigation aimed to id...
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| Format: | Article |
| Language: | English |
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Sciendo
2024-09-01
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| Series: | Fisheries & Aquatic Life |
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| Online Access: | https://doi.org/10.2478/aopf-2024-0014 |
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| author | Lucano Billy M. Feria Manuel A. Zatan Adrian E. Toledo Odalis E. Aguilar Jorge L. Diringer Benoit M. Castañeda Arnaldo E. |
| author_facet | Lucano Billy M. Feria Manuel A. Zatan Adrian E. Toledo Odalis E. Aguilar Jorge L. Diringer Benoit M. Castañeda Arnaldo E. |
| author_sort | Lucano Billy M. |
| collection | DOAJ |
| description | The emergence of diseases and the indiscriminate use of antibiotics have had deleterious consequences in the aquaculture industry. Consequently, the growing emphasis on eco-friendly alternatives has taken center stage, and probiotics have emerged as a notable solution. This investigation aimed to identify and characterize potential probiotic bacteria present in the gills and intestine of rainbow trout (Oncorhynchus mykiss (Walbaum)). A total of 29 isolates were selected for in vitro evaluation. The assays included morphological evaluation, catalase, oxidase, hemolysis, pathogen antagonism, antimicrobial susceptibility, protease, and amylase tests. Seven isolates exhibited antagonism against one of the three pathogens tested (Aeromonas hydrophila, Streptococcus agalactiae, and Lactococcus garvieae). None of the isolates showed resistance to the antibiotics ampicillin, florfenicol, or tetracycline. Furthermore, all isolates showed proteolytic but not amylolytic activity. The isolates with the best characteristics were identified as Lactococcus lactis (n=7) by 16S rRNA gene sequencing. |
| format | Article |
| id | doaj-art-fbcc627b45354288bb21682b84aa687a |
| institution | Kabale University |
| issn | 2545-059X |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Fisheries & Aquatic Life |
| spelling | doaj-art-fbcc627b45354288bb21682b84aa687a2025-02-10T13:25:25ZengSciendoFisheries & Aquatic Life2545-059X2024-09-0132315516510.2478/aopf-2024-0014Assessing the probiotic potential of Lactococcus lactis isolated from the intestine and gill of rainbow trout (Oncorhynchus mykiss) through in vitro analysisLucano Billy M.0Feria Manuel A.1Zatan Adrian E.2Toledo Odalis E.3Aguilar Jorge L.4Diringer Benoit M.5Castañeda Arnaldo E.61National University of Tumbes, Aquaculture Department, Tumbes/Tumbes, Peru(24001)2Pezbiotec SAC, Mz D Lote 24, Urb. Hermanos Carcamo, Piura/Piura, Peru (20009)2Pezbiotec SAC, Mz D Lote 24, Urb. Hermanos Carcamo, Piura/Piura, Peru (20009)2Pezbiotec SAC, Mz D Lote 24, Urb. Hermanos Carcamo, Piura/Piura, Peru (20009)2Pezbiotec SAC, Mz D Lote 24, Urb. Hermanos Carcamo, Piura/Piura, Peru (20009)3IncaBiotec SAC, Jr. Filipinas 212, Tumbes/Tumbes, Peru (24001)2Pezbiotec SAC, Mz D Lote 24, Urb. Hermanos Carcamo, Piura/Piura, Peru (20009)The emergence of diseases and the indiscriminate use of antibiotics have had deleterious consequences in the aquaculture industry. Consequently, the growing emphasis on eco-friendly alternatives has taken center stage, and probiotics have emerged as a notable solution. This investigation aimed to identify and characterize potential probiotic bacteria present in the gills and intestine of rainbow trout (Oncorhynchus mykiss (Walbaum)). A total of 29 isolates were selected for in vitro evaluation. The assays included morphological evaluation, catalase, oxidase, hemolysis, pathogen antagonism, antimicrobial susceptibility, protease, and amylase tests. Seven isolates exhibited antagonism against one of the three pathogens tested (Aeromonas hydrophila, Streptococcus agalactiae, and Lactococcus garvieae). None of the isolates showed resistance to the antibiotics ampicillin, florfenicol, or tetracycline. Furthermore, all isolates showed proteolytic but not amylolytic activity. The isolates with the best characteristics were identified as Lactococcus lactis (n=7) by 16S rRNA gene sequencing.https://doi.org/10.2478/aopf-2024-0014autochthonousgutlactic acid bacteriamicrobiotafish health |
| spellingShingle | Lucano Billy M. Feria Manuel A. Zatan Adrian E. Toledo Odalis E. Aguilar Jorge L. Diringer Benoit M. Castañeda Arnaldo E. Assessing the probiotic potential of Lactococcus lactis isolated from the intestine and gill of rainbow trout (Oncorhynchus mykiss) through in vitro analysis Fisheries & Aquatic Life autochthonous gut lactic acid bacteria microbiota fish health |
| title | Assessing the probiotic potential of Lactococcus lactis isolated from the intestine and gill of rainbow trout (Oncorhynchus mykiss) through in vitro analysis |
| title_full | Assessing the probiotic potential of Lactococcus lactis isolated from the intestine and gill of rainbow trout (Oncorhynchus mykiss) through in vitro analysis |
| title_fullStr | Assessing the probiotic potential of Lactococcus lactis isolated from the intestine and gill of rainbow trout (Oncorhynchus mykiss) through in vitro analysis |
| title_full_unstemmed | Assessing the probiotic potential of Lactococcus lactis isolated from the intestine and gill of rainbow trout (Oncorhynchus mykiss) through in vitro analysis |
| title_short | Assessing the probiotic potential of Lactococcus lactis isolated from the intestine and gill of rainbow trout (Oncorhynchus mykiss) through in vitro analysis |
| title_sort | assessing the probiotic potential of lactococcus lactis isolated from the intestine and gill of rainbow trout oncorhynchus mykiss through in vitro analysis |
| topic | autochthonous gut lactic acid bacteria microbiota fish health |
| url | https://doi.org/10.2478/aopf-2024-0014 |
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