Enhanced effect of radiofrequency ablation on HCC by siRNA-PD-L1-endostatin Co-expression plasmid delivered
Hepatocellular carcinoma (HCC) poses a significant clinical challenge due to high mortality and limited treatment options. Radiofrequency ablation (RFA) is commonly used but can be limited by tumor recurrence. This study explores the potential of combining RFA with an attenuated Salmonella strain ca...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | Translational Oncology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1936523325000506 |
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| author | Pengfei Chen Kun Li Jinwei Chen He Hei Jiaxin Geng Nannan Huang Mengyu Lei Huijie Jia Jianzhuang Ren Chenwang Jin |
| author_facet | Pengfei Chen Kun Li Jinwei Chen He Hei Jiaxin Geng Nannan Huang Mengyu Lei Huijie Jia Jianzhuang Ren Chenwang Jin |
| author_sort | Pengfei Chen |
| collection | DOAJ |
| description | Hepatocellular carcinoma (HCC) poses a significant clinical challenge due to high mortality and limited treatment options. Radiofrequency ablation (RFA) is commonly used but can be limited by tumor recurrence. This study explores the potential of combining RFA with an attenuated Salmonella strain carrying siRNA-PD-L1 and endostatin to enhance HCC treatment. In this study, an H22 subcutaneous tumor mouse model was used, with animals divided into five groups for treatment with a blank control, a blank Salmonella plasmid, RFA alone, siRNA-PD-L1-endostatin, or a combination of RFA and siRNA-PD-L1-endostatin. The combination therapy significantly reduced tumor growth, angiogenesis, and PD-L1/VEGF expression in tumor tissues post-RFA. Additionally, it induced tumor cell apoptosis, inhibited proliferation and migration, and increased the infiltration of T lymphocytes, granzyme B+ T cells, and CD86+macrophages within tumors. There was also a notable rise in T and NK cell populations in the spleen. In conclusion, combining RFA with siRNA-PD-L1-endostatin delivered by attenuated Salmonella synergistically enhances anti-tumor effects, boosts the anti-tumor immune response, and improves RFA efficacy for HCC. |
| format | Article |
| id | doaj-art-fbe18caac8f34ae38e2d35b5d46c2ba8 |
| institution | Kabale University |
| issn | 1936-5233 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Translational Oncology |
| spelling | doaj-art-fbe18caac8f34ae38e2d35b5d46c2ba82025-02-12T05:30:57ZengElsevierTranslational Oncology1936-52332025-03-0153102319Enhanced effect of radiofrequency ablation on HCC by siRNA-PD-L1-endostatin Co-expression plasmid deliveredPengfei Chen0Kun Li1Jinwei Chen2He Hei3Jiaxin Geng4Nannan Huang5Mengyu Lei6Huijie Jia7Jianzhuang Ren8Chenwang Jin9Department of Radiology, the First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Road, Xi'an, Shaanxi 710061, PR China; Department of Interventional Radiology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, PR ChinaDepartment of Interventional Radiology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, PR ChinaDepartment of Interventional Radiology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, PR ChinaDepartment of Radiology, the First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Road, Xi'an, Shaanxi 710061, PR ChinaXinxiang Engineering Technology Research Center of immune checkpoint drug for Liver-Intestinal Tumors, Xinxiang Medical University, Xinxiang, Henan, PR ChinaDepartment of Orthopedics, Zhengyang county traditional Chinese medicine hospital, Zhumadian, Henan, PR ChinaXinxiang Engineering Technology Research Center of immune checkpoint drug for Liver-Intestinal Tumors, Xinxiang Medical University, Xinxiang, Henan, PR ChinaXinxiang Engineering Technology Research Center of immune checkpoint drug for Liver-Intestinal Tumors, Xinxiang Medical University, Xinxiang, Henan, PR ChinaDepartment of Interventional Radiology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, PR ChinaDepartment of Radiology, the First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Road, Xi'an, Shaanxi 710061, PR China; Shaanxi Engineering Research Center of Computational Imaging and Medical Intelligence, 277 West Yanta Road, Xi'an, Shaanxi 710061, PR China; Corresponding author: Department of Radiology, the First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Road, Xi'an, Shan'xi 710061, PR China.Hepatocellular carcinoma (HCC) poses a significant clinical challenge due to high mortality and limited treatment options. Radiofrequency ablation (RFA) is commonly used but can be limited by tumor recurrence. This study explores the potential of combining RFA with an attenuated Salmonella strain carrying siRNA-PD-L1 and endostatin to enhance HCC treatment. In this study, an H22 subcutaneous tumor mouse model was used, with animals divided into five groups for treatment with a blank control, a blank Salmonella plasmid, RFA alone, siRNA-PD-L1-endostatin, or a combination of RFA and siRNA-PD-L1-endostatin. The combination therapy significantly reduced tumor growth, angiogenesis, and PD-L1/VEGF expression in tumor tissues post-RFA. Additionally, it induced tumor cell apoptosis, inhibited proliferation and migration, and increased the infiltration of T lymphocytes, granzyme B+ T cells, and CD86+macrophages within tumors. There was also a notable rise in T and NK cell populations in the spleen. In conclusion, combining RFA with siRNA-PD-L1-endostatin delivered by attenuated Salmonella synergistically enhances anti-tumor effects, boosts the anti-tumor immune response, and improves RFA efficacy for HCC.http://www.sciencedirect.com/science/article/pii/S1936523325000506Hepatocellular carcinomaRadiofrequency ablationRNA interferencePD-L1Endostatin |
| spellingShingle | Pengfei Chen Kun Li Jinwei Chen He Hei Jiaxin Geng Nannan Huang Mengyu Lei Huijie Jia Jianzhuang Ren Chenwang Jin Enhanced effect of radiofrequency ablation on HCC by siRNA-PD-L1-endostatin Co-expression plasmid delivered Translational Oncology Hepatocellular carcinoma Radiofrequency ablation RNA interference PD-L1 Endostatin |
| title | Enhanced effect of radiofrequency ablation on HCC by siRNA-PD-L1-endostatin Co-expression plasmid delivered |
| title_full | Enhanced effect of radiofrequency ablation on HCC by siRNA-PD-L1-endostatin Co-expression plasmid delivered |
| title_fullStr | Enhanced effect of radiofrequency ablation on HCC by siRNA-PD-L1-endostatin Co-expression plasmid delivered |
| title_full_unstemmed | Enhanced effect of radiofrequency ablation on HCC by siRNA-PD-L1-endostatin Co-expression plasmid delivered |
| title_short | Enhanced effect of radiofrequency ablation on HCC by siRNA-PD-L1-endostatin Co-expression plasmid delivered |
| title_sort | enhanced effect of radiofrequency ablation on hcc by sirna pd l1 endostatin co expression plasmid delivered |
| topic | Hepatocellular carcinoma Radiofrequency ablation RNA interference PD-L1 Endostatin |
| url | http://www.sciencedirect.com/science/article/pii/S1936523325000506 |
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